Table 1.

Characteristics of the experimental datasets. n is the number of mutated positions and k is the number of residues at each position

Dataset	Size of dataset	n	k	Theoretical size of sequence space	Length of protein sequence
Cyt P450	242	8	3	6561	464–466
GLP-2	31	31	2	2.147 billion	33
Enterotoxin	12	40	2	1099.5 billion	233
TNF	21	17	[2, 7, 4, 6, 2, 9, 9, 9, 9, 9, 2, 2, 2, 2, 6, 8, 7]	213.3 billion	157

The theoretical size of sequence space S is calculated as the product all k values for all mutated positions