Fig. 2.

TMZ-POH leads to mitochondria fission. a A549, SPC-A1 cells treated with TMZ, POH, TMZ + POH, TMZ-POH respectively were fixed and stained with anti-COX-IV antibody. Mitochondrial perimeter per cell was measured using the Fiji Image J program. b A549 cells treated with NAM, TMZ-POH respectively were fixed and stained with anti-TOM20 antibody. Mitochondrial perimeter per cell was measured using the Fiji Image J program. c Mitochondria structure in A549 cells treated with TMZ-POH or DMSO was observed by TEM. d The shape and area of mitochondria in above drug-treated cells were measured using Fiji Image J program