Fig. 7.

TMZ-POH down-regulates RAB7A via mevalonate pathway. a RAB7A activity was analyzed in lysates containing equal amounts of total proteins from A549 cells treated with TMZ-POH or not; (b, c) RAB7A expression in A549, SPC-A1, H460 and H520 cells (b); RILP expression in A549 and SPC-A1 cells (c) was detected by western blot when treated with TMZ-POH or not; (d) RAB7A in A549 treated with TMZ-POH for indicated concentration was analyzed by western blot; (e) A549 and SPC-A1 cells were treated with TMZ-POH with or without presence of MVL or GGOH, western blot analysis demonstrated RAB7A expression, ACTB as control