Figure 4.

Inhibition of the SAC Suppresses Imaginal Wing Disc Apoptosis Caused by Premature Loss of Cohesin

(A) Images of cleaved caspase-3 (CC3) immunofluorescence in controls (Rad21^TEV without TEV and mad2^P after HS), Rad21^TEV + TEV protease, and Rad21^TEV + TEV protease in mad2 mutant background after HS. The scale bar represents 100 μm.

(B) Quantification of CC3-positive area of the entire wing disc, in the indicated experimental conditions; n ≥ 5 independent discs per experimental condition; statistical analysis was performed using one-way ANOVA test.

(C) Representative images of CC3 immunofluorescence in control, san RNAi, and san and mad2 double RNAi. Scale bar indicates 100 μm.

(D) Quantification of CC3-positive area of the wing disc pouch, in control, san, and san and mad2 RNAi; n ≥ 4 independent discs per experimental condition; multiple comparison analysis was perform using a one-way ANOVA test.