Fig. 6.

Generation of long-lived MPER- and LACK-specific plasma cells in bone marrow promoted by sLACK and pLACK formulated MPER/liposome vaccine (A) Experimental timeline to define long-term bone marrow MPER- and LACK-specific ASCs. ELISPOT analysis was performed at 150dpi and examples of high density (1:50 ratio, peptide:lipid) and low density (1:1000) MPER or LACK/liposome reactive spots from pLACK- and sLACK-formulated liposomes as shown. (B) Quantification of MPER- (top) and LACK-specific ASCs (bottom) per million bone marrow cells (1:50, left columns; 1:1000, right columns) from sLACK (open bars) and pLACK (filled bars) formulated MPER/liposome. (C) Relative affinity of MPER- and LACK-specific bone marrow ASCs. ns = not significant and ***p < 0.001 by the unpaired Students t-test. Mean ±SEM shown for n = 5 mice (individual circles).