Key Resources Table
| Reagent/Material | Source | Identifier |
|---|---|---|
| Antibodies: | ||
| Alexa Fluora 594 Streptavidin | Biolegend | Cat #: 405240 |
| Alexa Fluroa 647 Streptavidin | Biolegend | Cat #: 405237 |
| Anti-Mouse CD8α, Clone: 53-6.7 | Biolegend | Cat #: 100725 |
| Anti-Mouse CD11b, Clone: M1/70 | Biolegend | Cat #: 101251 |
| Anti-Mouse CD11c, Clone: HL3 | BD Biosciences | Cat #: 553801 |
| Anti-Mouse CD11c, Clone: N418 | Biolegend | Cat #: 117346 |
| Anti-Mouse CD45.1, Clone: A20 | Biolegend | Cat #: 110726 |
| Anti-Mouse CD45.2, Clone: 104 | Biolegend | Cat #: 109807 |
| Anti-Mouse CD169, Clone: 3D6.112 | Biolegend | Cat #: 142419 |
| Anti-Mouse CD197 (CCR7), Clone: 4B12 | Biolegend | Cat #: 120116 |
| Anti-Mouse CD273 (PDL2), Clone: TY25 | BD Biosciences | Cat #: 560086 |
| Anti-mouse CD301b (MGL2), Clone: URA-1 | Biolegend | Cat #: 146806 |
| Anti-Mouse F4/80, Clone: BM8 | Biolegend | Cat #: 123131 |
| Anti-Mouse Lyve-1, Clone: ALY7 | eBioscience | Cat #: 53-0443-80 |
| Anti-Mouse Podoplanin, Clone: 8.1.1 | Biolegend | Cat #: 127406 |
| Anti-rabbit IgG, HRP | Cell Signaling | Cat #: 7074S |
| Anti-SHP2 | Cell Signaling | Cat #: 3397T |
| Anti-phospho SHP2 (Tyr 542) | Cell Signaling | Cat #: 3751S |
| Anti-Siglec1, biotinylated | R&D Systems | Cat #: BAF5610 |
| Anti-SIGN-R1, biotinylated | R&D Systems | Cat #: BAF1836 |
| Anti-Src | Cell Signaling | Cat #: 2108S |
| Anti-phospho Src (Tyr 416) | Cell Signaling | Cat #: 2101S |
| Biotinylated Anti-mouse MCP-2/CCL8 Antibody | R&D Systems | Cat #: BAF790 |
| Human CCL1-AlexaFluor647 | Almac | Cat #: CAF-7 |
| Fixable Viability Dye eFluor 780 | Invitrogen | Cat #: 65-0865-14 |
| Mouse CCL1/I-309/TCA-3 Biotinylated Antibody | R&D Systems | Cat #: BAF845 |
| TruStain fcX anti-mouse CD16/32, Clone: 93 | Biolegend | Cat #: 101320 |
| Critical Commercial Assays: | ||
| Mouse IL-4 ELISA MAX Standard | Biolegend | Cat #: 431103 |
| Mouse IFN-g ELISA MAX Standard | Biolegend | Cat #: 430803 |
| Mouse IL-13 ELISA Ready-SET-Go! | eBioscience | Ref #: 88-7137-77 |
| RNeasy Plus Micro Kit (50) | Qiagen | Cat #: 74034 |
| RNeasy Plus Mini Kit (50) | Qiagen | Cat #: 74134 |
| Naïve CD4+ T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat #: 130-104-453 |
| CD11c Microbeads UltraPure mouse | Miltenyi Biotec | Cat #: 130-108-338 |
| Experimental Models: Organisms and Strains: | ||
| Mouse: C57Bl/6 | Charles River Laboratory | Strain No.: 027 |
| Mouse: C57Bl/6 | The Jackson Laboratory | Strain No.: 000664 |
| Mouse: B6(FVB)-Mgl2tm1.1(HBEGF/EGFP)Aiwsk/J (CD301b-DTR) | The Jackson Laboratory | Strain No.: 023822 |
| Mouse: Ccr8−/− | Chensue et al., 2001 | N/A |
| Mouse: Ccl8−/−Ccl12−/− | Tsou et al., 2007 | N/A |
| Mouse: Kaede+ | Tomura et al., 2008 | N/A |
| Mouse: Ccr7−/− | The Jackson Laboratory | Strain No.: 006621 |
| Mouse: B6.Cg-Tg (TcraTcrb)425Cbn/J (OT2m) | The Jackson Laboratory | Strain No.: 004194 |
| Mouse: B6.SJL-Ptprc Pepc/BoyJ (CD45.1) | The Jackson Laboratory | Strain No.: 002014 |
| Mouse: REC8 | Cyagen Biosciences, this manuscript | N/A |
| Oligionucleotides | ||
| mCcl1 FWD (5’-GGCTGCCGTGTGGATACAG-3’) | This manuscript | N/A |
| mCcl1 REV (5’-AGGTGATTTTGAACCCACGTTT-3’) | This manuscript | N/A |
| mCcl2 FWD (5’-TGGCTCAGCCAGATGCAGT-3’) | This manuscript | N/A |
| mCcl2 REV (5’-TTGGGATCATCTTGCTGGTG-3’) | This manuscript | N/A |
| mCcl7 FWD (5’-TGGGAAGCTGTTATCTTCAAGACA’3’) | This manuscript | N/A |
| mCcl7 REV (5’-CTCGACCCACTTCTGATGGG-3’) | This manuscript | N/A |
| mCcl8 FWD (5’-GCGAGTGCTTCTTTGCCTG-3’) | This manuscript | N/A |
| mCcl8 REV (5’ TCTGGCCCAGTCAGCTTCTC-3’) | This manuscript | N/A |
| mCcl12 FWD (5’-GCTGGACCAGATGCGGTG-3’) | This manuscript | N/A |
| mCcl12 REV (5’-CCGGACGTGAATCTTCTGCT-3’) | This manuscript | N/A |
| REC8 genotyping primer P1 forward (5’-GAACACAGCAGCAGAAAGAACAC-3’) | This manuscript | N/A |
| REC8 genotyping primer P1 reverse (5’-CATGGCGTGTAATATGAGAGACGC-3’) | This manuscript | N/A |
| REC8 genotyping primer P2 forward (5’-GTAATAAATGGATGCCCTGCGTAAG-3’) | This manuscript | N/A |
| REC8 genotyping primer P2 reverse (5’-ACCTGTCAAGAATGGTGCCAG-3’) | This manuscript | N/A |
| REC8 genotyping primer P3 forward (5’-ATCAGTCAGGTACATAACCTCGAG-3’) | This manuscript | N/A |
| REC8 genotyping primer P3 reverse (5’-AGTCCATAACCTTCCTCCACG-3’) | This manuscript | N/A |
| REC8 genotyping primer P4 forward (5’-GATTGGTATCTGCTTCTGCACTTG=3’) | This manuscript | N/A |
| REC8 genotyping primer P4 reverse (5’-GTAGATGAACTCGCCGTCCTG-3’) | This manuscript | N/A |
| REC8 genotyping primer P5 forward (5’-CCTTCTCACCACAGCCACTTC-3’) | This manuscript | N/A |
| REC8 genotyping primer P5 reverse (5’-TGAGTTTGGACAAACCACAAC-3’) | This manuscript | N/A |
| REC8 genotyping primer P6 forward (5’-TGCTTCATGTGGTCGGGGTA-3’) | This manuscript | N/A |
| REC8 genotyping primer P6 reverse (5’-GCTTTCATTTCCCATTAGGCACA-3’) | This manuscript | N/A |
| Chemicals, Peptides, & Recombinant Proteins: | ||
| Macrophage depletion kit of Clodrosome and Encapsome | Encapsula Nano Sciences | Cat #: 8901 |
| Saponin from quillaja bark | Sigma-Aldrich | Cat #: S7900-25G |
| Recombinant Mouse CCL1/TCA-3 | R&D Systems | Cat #: 845-TC |
| Recombinant Mouse CCL21/6Ckine | R&D Systems | Cat #: 457-6C-025 |
| Recombinant Murine MCP-3 (CCL7) | PeproTech | Cat #: 250-08 |
| Recombinant Murine MCP-2 (CCL8) | PeproTech | Cat #: 250-14 |
| Recombinant Mouse CCL2/JE/MCP-1 | R&D Systems | Cat #: 479-JE-010 |
| Recombinant Mouse CCL11/Eotaxin | R&D Systems | Cat 3: 420-ME |
| TRIzol Reagent | Invitrogen | Ref #: 15596018 |
| Collagenase P | Sigma-Aldrich | Cat #: 11213857001 |
| Dispase II | Sigma-Aldrich | Cat #: D4693-1G |
| DNase I, grade II | Sigma-Aldrich | Cat #: 10104159001 |
| Liberase DH | Sigma-Aldrich | Cat #: 5401054001 |
| Streptavidin/Biotin Blocking Kit | Vector Lab | Cat #: SP-2002 |
| Papain | Calbiochem | Cat: 5125 |
| Ovalbumin | Sigma-Aldrich | Cat: A5503 |
| LPS | Invivogen | Cat: tlrl-eklps |
| Polyinosinic-polycytidylic acid | Invivogen | Cat:tlrl-picw |
| PBS | Corning | Ref #: 21-031-CV |
| PP2 | Sigma-Aldrich | Cat #: 529573-1MG |
| Indo-1, AM | Invitrogen | Cat #: I1223 |
| RBC Lysis buffer | Sigma-Aldrich | Cat #: R7757 |
| 0.5 M EDTA, pH 8.0 | Gibco | Cat #: 15575-038 |
| OCT Compound | Tissue-Tek | Cat #: 4583 |
| RPMI 1640, 1X | Corning | Ref #: 15-040-CV |
| Fetal Calf Serum (FCS) | Atlanta Biologicals | Cat #: S11550 |
| MultiScribe Reverse Transcriptase | Thermo Fisher Scientific | Ref #: 4311235 |
| GeneAMP dNTP mix with dTTP | Thermo Fisher Scientific | Ref #: N8080260 |
| MgCl2 Solution 25 mM | Thermo Fisher Scientific | Ref #: 4486224 |
| 10× PCR Buffer II | Thermo Fisher Scientific | Ref #: 4486220 |
| Oligio d(T)16 | Thermo Fisher Scientific | Ref #: 100023441 |
| Random Hexamers 50 μM | Thermo Fisher Scientific | Ref #: 100026484 |
| RNase Inhibitor | Thermo Fisher Scientific | Ref #: 100021540 |
| FastStart Essential DNA Green Master | Roche | Cat #: 25595200 |
| Software: | ||
| Prism v 7.0c | GraphPad | https://www.graphpad.com/demos |
| Zen Blue | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Imaris | Bitplane | http://www.bitplane.com/download |
| Softmax Pro | Molecular Devices | https://www.moleculardevices.com/systems/microplate-readers/softmax-pro-7-software |
| BD FACS Diva 8 | BD Biosciences | http://www.bdbiosciences.com/us/instruments/clinical/software/flow-cytometry-acquisition/bd-facsdiva-software/m/333333/overview |
| FlowJo (version 10) | Tree Star | https://www.flowjo.com/solutions/flowjo/downloads |