Fig. 2.

RSG alleviates PA-induced lipid accumulation in Sertoli cells. TM4 cells (a and b) and primary mouse Sertoli cells (c and d) were pre-treated with 20 μM RSG for 2 h, and then treated with 0.2 or 0.4 mM PA for 24 h. a and b ORO staining of TM4 cells (a) and quantification of neutral lipids (b). c and d ORO staining of primary mouse Sertoli cells (c) and quantification of neutral lipids (d). Data are presented as the mean ± standard deviation of three independently prepared samples, each with three measurements. Scale bar, 100 μm.^**P < 0.01 vs. control group; ^$$P < 0.01 vs. 0.2-mM PA group; ^##P < 0.01 vs. 0.4 mM-PA group. RSG rosiglitazone, PA palmitic acid, ORO oil red O