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. 2018 Oct 10;11:367. doi: 10.3389/fnmol.2018.00367

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Copyright © 2018 Garcia-Pradas, Gleiser, Wizenmann, Wolburg and Mack.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Newly formed axons are located on the innermost NFL. (a) Labeled by antibodies for doublecortin (DCX; green), new axons are found close to the inner limiting membrane. (b) These DCX axons originate from maturing GCs next to peripheral growth zone (PGZ) where proliferating cells are labeled by antibodies for proliferating cell nuclear antigen (PCNA). Tight junctions in the NFL are labeled by ZO-1. (c,d) Electron micrographs reveal that unmyelinated nerve fibers are separated from the vitreous (V) and surrounded by Müller cell endfeet (MC). New axons are indicated by arrows in (a–c). Scale bars 20 μm in (a,b), 1 μm in (c), and 200 nm in (d).