Table 1.
List of antibodies used.
| Primary Antibody | Specificity/Immunogen | Source (catalog number) | Host | Dilution |
|---|---|---|---|---|
| Anti-claudin-1 | C-terminal of human/mouse claudin-1 | Thermo Fisher Scientific (71-7800) | Rabbit | 1:100 |
| Anti-claudin-3 | C-terminal of mouse claudin-3 | Thermo Fisher Scientific (34-1700) | Rabbit | 1:100 |
| Anti-claudin-19 | C-terminal of mouse claudin-19 | A gift from Dr. M. Furuse | Rabbit | 1:100 |
| 3A10 anti-neurofilament-associated antigen | Neurofilament associated protein from chick spinal cord | Iowa Hybridoma Bank | Mouse | 1:100 |
| Anti-ZO-1 | Aa 334-634 of human ZO-1 | Thermo Fisher Scientific (33-9100) | Mouse | 1:100 |
| Anti-myelin basic protein | Aa 82-87 | Abcam ab7349 | Rat | 1:125 |
| Anti-Doublecortin | C-terminal of human doublecortin | Santa Cruz Biotechnology sc-8066 | Goat | 1:100 |
| Anti-proliferating cell nuclear antigen (PCNA) | Rat PCNA fusion protein | DAKO M0879 | Mouse | 1:300 |
| Anti-aquaporin-4 | Aa 244-323 at C-termial of human AQP4 | Santa Cruz Biotechnology sc-20812 (H80) | Rabbit | 1:100 |
| Anti GFAP | Porcine GFAP | Santa Cruz Biotechnology sc-58766 | Mouse | 1:100 |
| Anti-proteolipid protein (PLP-1) | N-terminal of PLP1 | Thermo Fisher Scientific (PA5-40788) | Rabbit | 1:100 |
| Secondary Antibody | Source | Host | Dilution | |
| Anti-rabbit Alexa 488 | Thermo Fisher Scientific | Goat | 1:400 | |
| Anti-mouse Alexa 546 | Thermo Fisher Scientific | Goat | 1:400 | |
| Anti-rabbit Alexa 546 | Thermo Fisher Scientific | Goat | 1:400 | |
| Anti-goat Alexa 488 | Thermo Fisher Scientific | Donkey | 1:400 | |
| Anti-mouse Alexa 546 | Thermo Fisher Scientific | Donkey | 1:400 | |
| Anti-rat Alexa 546 | Thermo Fisher Scientific | Goat | 1:400 |