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. 2018 Oct 10;9:2341. doi: 10.3389/fimmu.2018.02341

Figure 4.

Figure 4

TIGIT is primarily expressed on CD226+ NK cells in HIV-infected individuals. (A) A representative flow cytometry plot showing the expression of CD226 on total NK cells in HIV-infected and HC individuals. (B) Comparison of the percentages of CD226+ NK cells in HIV-infected (n = 26) and HC (n = 38) groups. (C) A representative flow cytometry plot showing the proportion of IFN-γ producing NK cells after treatment with 20 μg/mL anti-human CD226 antibodies or purified mouse IgG (as a negative control) from HIV-infected individuals. The expression of IFN-γ was gated according to an isotype control. (D) Paired comparisons of the proportion of IFN-γ producing NK cells from HIV-infected individuals (n = 8) after treatment with 20 μg/mL anti-human CD155 antibodies or purified anti-mouse IgG (as a negative control). (E) Comparison of TIGIT expression on CD226+ NK cells in the HIV (n = 13) and HC (n = 9) groups. (F) Paired comparisons of the expression of TIGIT on CD226+ NK cells unstimulated or stimulated with rIL-10, rIL-12 + rIL-15, or rTGF-β. A Mann-Whitney U test was used for comparisons between two groups. A Wilcoxon matched-pairs signed-rank test was used for paired-group comparisons. p < 0.05 was considered significant.