Fig. 2.

The impact of CLDN2 on the proliferation and metastasis ability of U2OS cells in vitro. a Detection of CLDN2 expression in the U2OS cells by Western blotting. b The corresponding statistical analysis of protein expression. c Detection of CLDN2 expression in the U2OS cells by immunofluorescence. d The growth curve of the U2OS cells was drawn by the CCK-8 method. e The abilities of U2OS cells to form colonies were detect by colony formation assay. f The cell cycle distribution of U2OS cells was analyzed using flow cytometry. g The percentage of cells with S phase DNA content from U2OS cells. h The transwell chamber method was utilized to detect the invasive ability of the U2OS cell line in vitro. i The wound healing assay was utilized to detect the migration ability of the U2OS cell line in vitro. *P < 0.05, **P < 0.01, compared with the control group compared with the vector group