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. 2018 Oct 16;37:252. doi: 10.1186/s13046-018-0927-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Biological role of miRNA-302a/d in HCC in vitro. qRT-PCR measurement of the levels of miRNA-302a/d mRNA in HepG2 and Huh7 cells before and after knockdown (a) or overexpression (b) of miRNA-302a/d. Data shown are the means ± SD of three independent experiments. c, Tumor sphere assays of HepG2 and Huh7 cells before and after knock down of miRNA-302a/d. d, HepG2 and Huh7 cell counts in 96-well plate after transfection with negative control or miRNA-302a/d knockdown plasmids at the indicated day. e, HepG2 and Huh7 cell apoptosis was examined by flow cytometry analysis before and after knockdown of miRNA-302a/d. f, Tumor sphere assays of HepG2 and Huh7 cells before and after overexpression of miRNA-302a/d. g, HepG2 and Huh7 cell counts in 96-well plate after transfection with negative control or miRNA-302a/d overexpression plasmids at the indicated day. h, HepG2 and Huh7 cell apoptosis was examined by flow cytometry analysis before and after overexpression of miRNA-302a/d. Statistical analyses were performed with one-way ANOVA (*P < 0.05, **P < 0.01, and ***P < 0.001 vs. normal)