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. 2017 Dec 18;28(2):90–99. doi: 10.1093/glycob/cwx097

Fig. 2.

Fig. 2.

Pn3Pase identification and domain schematic of amino acid sequence. (A) Amino acid sequence and accession of Pn3Pase. Underlined are tryptic peptides identified with high confidence by proteome discoverer software. (B) Purification is His-tagged recombinant Pn3Pase by Nickel-NTA column. Lane (1) uncaptured flow-through, (2) 5× concentrated wash, (3) elution. (C) Separation of recombinant Pn3Pase depolymerized tritium radioisotope labeled Pn3P by size exclusion chromatography, measured by counts per minute in each 1 ml fraction. (D) Dot blot of recombinant Pn3Pase depolymerized cold Pn3P probed with Pn3P monoclonal antibody. (E) Schematic of predicted domains of Pn3Pase by InterPro.