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. 2017 Dec 18;28(2):90–99. doi: 10.1093/glycob/cwx097

Fig. 5.

Fig. 5.

Activity assessment of Pn3Pase. (A) Time course assay of recombinant Pn3Pase depolymerized tritium radioisotope labeled Pn3P separated by size exclusion chromatography, measured by counts per min in each 1 ml fraction. (B) Optimization of recombinant Pn3Pase in three different buffer conditions, measured by concentration of glucuronic acid reducing end generated in μg/ml. (C) Metal dependence determination with Mg2+ and Ca2+ reaction supplements, measured by concentration of glucuronic acid reducing end generated in ug/ml. (D) Temperature dependence of recombinant Pn3Pase, measured by concentration of glucuronic acid reducing end generated in μg/ml. Statistical significance was determined with the two-tailed Student t-test, ***P < 0.001, **P < 0.01; ns, not significant.