Figure 3.

Case 1: (a–c, d–f) The distribution of PrP (a, d), p‐tau (b, e), and Aβ (c, f) in the temporal cortex (a–c) and hippocampus (d–f) is shown. The photos are taken from an identical area for a–c and d–f. P‐tau (b&e, AT8‐immunostain) seems considerably overlapped with PrP (a&d, 3F4‐immunostain), whereas Aβ (c&f, 4G8‐immunostain) is completely absent (Original magnification: a–c, ×40; d–f, ×20). (g) P‐tau‐positive dystrophic neurites (DNs) are aggregated around a PrP‐plaque (AT8‐immunostain, temporal cortex, ×600). (h) A fraction of DNs are argyrophilic (Gallyas silver stain, temporal cortex, ×600). (i) By double‐immunostaining with AT8 and PrP‐N, the Colocalization of p‐tau with PrP‐plaques is confirmed (p‐tau, red; PrP, brown. Occipital cortex, ×600)