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. 2018 Oct 10;9:2319. doi: 10.3389/fimmu.2018.02319

Figure 2.

Figure 2

Formulation of OVA/CpG-ODN with Coa-ASC16 optimizes humoral and CD8+ T-cell responses independently of IL-6. WT or Il6−/− mice were immunized with OVA/Coa-ASC16, OVA/CpG-ODN, OVA/CpG-ODN/Coa-ASC16 (on days 0, 7, and 14) or OVA/CFA (days 0, 15, and 30). (A,C) In vivo killing assay and (B,D) ex vivo IFN-γ secretion by splenocytes after stimulation with SIINFEKL peptide determined by ELISA on day 21. (E) Titers of OVA-specific IgG1 and IgG2c in plasma determined by ELISA on day 21. (F) WT mice. Avidity OVA-specific IgG in plasma determined by ELISA using KSCN elution seven days after the last immunization. The data show the mean ± SEM of individual values (3-4 mice/treatment group in each experiment) and are representative of two independent experiments performed. *p < 0.05, **p < 0.01, ***p < 0.001.