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. 2018 Oct 10;9:2319. doi: 10.3389/fimmu.2018.02319

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Copyright © 2018 Chiodetti, Sánchez Vallecillo, Dolina, Crespo, Marin, Schoenberger, Allemandi, Palma, Pistoresi-Palencia, Morón and Maletto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The formulation of OVA/CpG-ODN with Coa-ASC16 enhances in vivo co-uptake of OVA and CpG-ODN by DCs. C57BL/6 mice were immunized in both hind limbs with Alexa Fluor 647-OVA/Alexa Fluor 488-CpG-ODN or Alexa Fluor 647-OVA/Alexa Fluor 488-CpG-ODN/Coa-ASC16. Seventy-two h later, cell suspension from inguinal LN were analyzed by flow cytometry. (A) Percentage and (B) absolute number of total CD11c⁺ DCs. (C) Percentage and (D) absolute number of total OVA⁺ CD11c⁺ DCs. (E) Percentage and (F) absolute number of total CpG-ODN⁺ CD11c⁺ DCs. (G) Percentage and (H) absolute number of OVA⁺ or CpG-ODN⁺ simple positive and OVA⁺ CpG-ODN⁺ double positive within CD11c⁺ DCs. The data show the mean ± SEM of individual values (3-4 mice/treatment group in each experiment) and are representative of two independent experiments performed. n.s., not significant, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.