. 2017 Aug 29;20(Suppl 6):21761. doi: 10.7448/IAS.20.7.21761

Table 1.

Steps in establishing final HIV‐1 infection status of 102 infants with non‐negative birth PCR results

First PCR result		Second PCR result			Earliest VL result			Final HIV‐1 infection status,^† N (%)
Birth HIV‐1 PCR test	n	Result	n	Age (days)^‡	n	VL (log RNA copies/ml)^‡	Age (days)^‡	Positive	Uncertain	Negative	LTFU
Positive	78	Positive	68	2 (1–9)	66	4.48 (3.4–5.4)	2 (1–8)	68
		Indeterminate	4	4, 4, 8, 40	4	<1.3^e, 2.58, 4.09, 4.56	68, 8, 4, 4	3	1^e
		Not tested	6		3	4.30, 5.04, 6.62	1, 4, 94	3			3
		Total birth HIV‐1 PCR positive results				74 (95)	1 (1)		3 (4)
Indeterminate	24	Positive	5	6 (6–12)	4	2.29, 2.96, 3.05, 4.45	12, 0, 6, 1	5
		Indeterminate	7	8 (6–24)	6	TNDⁱ, 3.1 (3.0–3.2)	2 (1–8)	4^a ^– ^d	2^g,i		1
		Negative	11	7 (3–11)	10	TND (n = 9), 2.82^f	8 (4–10)		2^f,h	8	1
		Not tested	1		0						1
		Total birth HIV‐1 PCR indeterminate results				9 (38)	4 (17)	8 (33)	3 (13)
Total, n = 102								83 (81)	5 (5)	8 (8)	6 (6)

†Positive HIV‐1 infection status was defined as two positive virological results from samples taken at two different time points. Negative HIV‐1 infection status was defined as an isolated indeterminate result followed by at least two negative virological results taken at two different time points whilst not on combination ART. Uncertain HIV infection status was defined as initial non‐negative virological results that did not meet either of the above criteria. Lost to follow‐up (LTFU) was defined as insufficient follow‐up testing to meet the above criteria.

‡Individual results are displayed for ≤4 cases and median (interquartile range (IQR)) for ≥5 cases.

PCR: polymerase chain reaction; VL: viral load; TND: target not detected.

The superscripts ^a ^– ^d in column Positive and ^e ^– ⁱ in column Uncertain indicate the cases presented in more detail in Figure 1(a,b), respectively.