(A) Frequency of GC TFH (CD4+ CD44hi CXCR5+ PD-1hi) cells in control or α-NK1.1 mice twelve days post-immunization with NP-KLH in alum.
(B) Total number of GC TFH day 12 post-immunization in spleen.
(C and D) Frequency of germinal center B (CD19+B220+Fas+GL-7+) cells in the spleens of control or α-NK1.1 mice day 12 post-immunization (C) and total number of GC B cells in spleen day 12 post-immunization (D). Data were analyzed via Student’s t test; n = 9–10 mice/group. Experiments were repeated 3 times.