(A) Total number of mutations in unique VH186.2 BCR sequences isolated from spleens of control or α-NK1.1 mice day 12 post-immunization with NP-KLH in alum. Data were analyzed via Mann-Whit-ney with median displayed; n = 5–6 mice/group.
(B) The proportion of unique VH186.2 sequences from (A) containing the W33L mutation in control and α-NK1.1 mice.
(C) The frequency of either replacement or silent mutations at all positions within the VH186.2 sequence between control or α-NK1.1 mice. Experiments were repeated 2 or 3 times.