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. 2018 Oct 17;13(10):e0205608. doi: 10.1371/journal.pone.0205608

Fig 3. CGCG elements promote bidirectional gene expression in the LuBiDi reporter system.

Fig 3

A) The structure of the LuBiDi reporter construct. B) One copy of the TCTCGCGAGA motif inserted in the LuBiDi construct was sufficient to activate the expression of both firefly and Renilla reporters. Flank-exchanged (AGACGCGTCT), G→T transversion mutation (TCTCTCGAGA), or an insertion mutation in the middle of CGCG (TCTCGACGAGA) abolished the dual activation. C) The frequency of common CGCG element sequence variants in the human genome. D) The bidirectional promoter activity of selected naturally-occurring CGCG element variants. E) The effect of CGCG element copy number (TATCGCGAGA motif) in LuBiDi on reporter activity. F) Corresponding transcript levels from reporters in panel E.