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. 2018 Oct 17;13(10):e0205608. doi: 10.1371/journal.pone.0205608

Fig 5. CGCG elements in CGI promoters drive gene expression.

Fig 5

A) The bidirectional promoter driving the POLR1C/YIPF3 genes (left panel) contains a conserved CGCG element between annotated TSSs (middle), which was cloned and tested for bidirectional promoter activity in LuBiDi. Right panel- the promoter insert orientation is defined by a large grey arrow, with the WT CGCG element flank orientation represented by ◊, and the flank-exchanged variants represented by ⊳⊲. B) The ZZZ3 promoter contains three CGCG elements. Although this promoter is annotated as unidirectional, the GRO-Cap analysis indicated associated divergent transcripts on the opposite strand. C) The promoter of PRDX1 gene contains both a TATA-box (⊳) and a CGCG element. D) Start-seq data analysis of CGCG elements in the mouse genome. The results suggest that CGCG elements are positioned in the nucleosome depleted regions between sense and anti-sense TSS. E) CGCG elements occur mostly within 50bp of sense and anti-sense Start-seq TSSs. Sense and anti-sense TSS occur in Py/Pu dinucleotide motif where Pu is the putative +1 nucleotide. Data are represented as the mean of three replicates +/- SD.