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. 2018 Oct 17;13(10):e0204998. doi: 10.1371/journal.pone.0204998

Fig 2. QRT-PCR validation of 7 miRNAs.

Fig 2

(A) The small RNA-Seq log2 value (expression ratios of S03-RPKM/S04-RPKM) and the qRT-PCR log2 value (expression ratios of S03/S04) of three important miRNAs (bna-miR156e, conservative-chrA05-439469 and unconservative-chrCnn-random-3253054) which the expression abundance was extremely high in two libraries (S03 and S04). (B) The small RNA-Seq log2 value (expression ratios of S03-RPKM/S04-RPKM) and the qRT-PCR log2 value (expression ratios of S03/S04) of two known miRNAs (bna-miR169m and bna-miR6029), one conservative miRNA (conservative-chrC07-2175567) and one novel miRNA (unconservative-chrC03-1462878) highly differentially expressed between S03 and S04. (C) Correlation analysis of the miRNA expression ratios obtained from the qRT-PCR and small RNA-seq data of 7 miRNAs (p-value <0.01).