Supplementary Fig 4.

Protein abundance of EGFR, Ras, and c-Fos in A431 cells. A431 cells were lysed with BlastR lysis buffer. Sample lysate, as well as EGFR, Ras, and c-Fos recombinant proteins were separated by SDS-PAGE. Samples were analyzed by western blot using EGFR, Ras, and c-Fos antibodies. Densitometic analysis of recombinant EGFR protein was used to establish a standard curve, and concentration of EGFR in A431 cells was determined based on normalization to that standard curve. Ras and c-Fos concentrations were determined using the same method with their respective standard curves.

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