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. 2018 Oct 17;4(10):eaau1780. doi: 10.1126/sciadv.aau1780

Fig. 3. Poly-Oxime gel limits AChE inhibition after exposure to a lethal dose of MPT in vivo.

Fig. 3

(A) Coat on the dorsal side of rats was clipped 1 day before the exposure. A dose of MPT (150 mg/kg) was applied on the skin either directly or in the presence of the sham gel (220 mg per animal) or poly-Oxime gel (220 mg per animal) layer. (B) Active AChE in the blood was quantified using Ellman’s assay. Direct exposure of MPT significantly reduced the active AChE in the blood. Sham gel could not limit MPT-induced AChE inhibition, while poly-Oxime gel deactivated MPT before entering into the skin, therefore reducing the MPT-induced AChE inhibition. (C to F) On day 5, rats were sacrificed, tissue was collected, and the amount of active AChE was quantified. Dermal exposure of MPT either directly on the skin or in the presence of sham gel led to the decrease in the active AChE in all tissues such as brain, heart, liver, and lung, while poly-Oxime gel significantly reduced this MPT-mediated deactivation of AChE. Data in (B) to (F) are means ± SD (n = 6 rats per group); P values were determined by one-way ANOVA with Tukey post hoc test. *P < 0.05, **P < 0.01, ****P < 0.0001.