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. 2018 Oct 5;7:e38611. doi: 10.7554/eLife.38611

Figure 1. The malonylation percentage of DTGs is highly uniform across treatments and developmental stages.

(A) Structures of 17-DTGs and their malonylation options. R1 could be glucose, rhamnose or hydrogen. R2 could be rhamnose or hydrogen. NaMaT indicates malonyltransferase in N. attenuata. (B) Relative abundance (mean + SE; n = 5 – 6) of different malonylated DTGs in N. attenuata leaves, after herbivory elicitation by immediately applying M. sexta oral secretions to freshly created leaf puncture wounds (W + OS), in comparison to the same nodal positions of plants without any treatment (Con). Inset: Total DTG abundance in control and W + OS elicited leaves. (C) Total DTGs in different stages of floral development. The formula (D) describes how malonylation percentage was calculated. (E, F) Malonylation percentage (mean + SE; n = 5 – 6) of DTGs in leaves treated by W + OS (D) or different developmental flower stages (E). Asterisks indicate significant differences between controls and treatments (*p<0.05; ***p<0.001; Student’s t-tests). Different letters indicate significant differences among floral developmental stages (p<0.05, one-way ANOVA followed by Tukey’s HSD post-hoc tests).

Figure 1—source data 1. DTG profiles and malonylation percentages under different treatments and developmental stages.
elife-38611-fig1-data1.xlsx (10.4KB, xlsx)
DOI: 10.7554/eLife.38611.006
Figure 1—source data 2. DTG profiles and malonylation percentages across different tissues.
elife-38611-fig1-data2.xlsx (11.2KB, xlsx)
DOI: 10.7554/eLife.38611.007

Figure 1.

Figure 1—figure supplement 1. Structural diversity of N. attenuata DTGs, their diagnostic molecular ion [M + Na]+ and retention times.

Figure 1—figure supplement 1.

Figure 1—figure supplement 2. The DTG malonylation percentage is remarkably uniform across different tissues.

Figure 1—figure supplement 2.

Fourteen tissues were collected from 28- and 50-d-old N. attenuata plants for DTGs analysis. Different tissues are represented by the symbols in (A). Relative abundance of different malonylated DTGs, including core (B), monomalonylated (C), dimalonylated (D), trimalonylated (E), and total DTGs (F). Malonylation percentage (G) was calculated based on DTG abundance. CV in each histogram represents the coefficient of variation of different malonylated DTGs and malonylation percentage. Asterisk indicates CV of (G) is an outlier based on the 3X interquartile range.