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. 2018 Oct 5;7:e38611. doi: 10.7554/eLife.38611

Figure 4. Silencing NaMaT1 influences DTG profiles and malonylation percentages across all tissues, but alters JAs and IAA specifically in flowers.

S1 leaves from early elongated VIGS plants were treated with 20 µL lanolin paste (A, leaf control) or 150 µg MeJA in lanolin paste (B, leaf MeJA treated) and samples were harvested after 3 days. The inflorescence (C), style and stigma (D) were harvested from flowering plants. Relative abundance of different malonylated DTGs (first column) were analyzed from upper samples. Malonylation percentage (second column) was calculated based on the DTG data using the formula (Figure 1). Jasmonates (JAs, third column) and IAA (fourth column) were analyzed from the same samples as those used for DTG quantification. N.D. indicates compounds which were not detected because of low concentrations. Asterisks above each column indicate significant differences between EV and VIGS-MaT1 plants (*p<0.05; **p<0.01; ***p<0.001; Student’s t-test).

Figure 4—source data 1. Silencing NaMaT1 influences DTG profiles and malonylation percentages across all tissues, but alters JAs and IAA specifically in flowers.
elife-38611-fig4-data1.xlsx (12.3KB, xlsx)
DOI: 10.7554/eLife.38611.022
Figure 4—source data 2. DTG profiles in VIGS plants having irGGPPS, irAOC, or EV backgrounds.
elife-38611-fig4-data2.xlsx (19.1KB, xlsx)
DOI: 10.7554/eLife.38611.023
Figure 4—source data 3. IAA and DTGs accumulate in high concentrations in the gynoecium.
elife-38611-fig4-data3.xlsx (11KB, xlsx)
DOI: 10.7554/eLife.38611.024

Figure 4.

Figure 4—figure supplement 1. DTG profiles in VIGS plants having irGGPPS, irAOC, or EV backgrounds.

Figure 4—figure supplement 1.

(A) Individual DTGs abundance (mean + SE; n = 9) in the styles of VIGS-MaT1 and VIGS-EV in the WT background. (B) Individual DTGs abundance (mean + SE; n = 9) in the inflorescences of VIGS-MaT1 and VIGS-EV in the background of irGGPPS and EV plants. Insert: malonylation percentages (mean + SE; n = 9) of DTGs. (C) DTGs with the same number of malonyl moieties were summed using data from (B). (D) Individual DTGs abundance (mean + SE; n = 3) in the styles of VIGS-MaT1 and VIGS-EV in background of irAOC and EV plants. (E) DTGs with the same number of malonyl moieties were summed using data from (D). Different letters indicate significant differences between genotypes (p<0.05, one-way ANOVA by Tukey’s HSD post-hoc tests). The statistic results for individual compounds are provided in the source data files.
Figure 4—figure supplement 2. IAA and DTGs accumulate in high concentrations in the gynoecium.

Figure 4—figure supplement 2.

S1 leaves and flower buds (one day before anthesis) were collected from flowering plants. (A) Tissues are represented by symbols as indicated. (B) IAA levels (mean + SE; n = 5) were analyzed from indicated tissues. DTG profiles (C) and their malonylation percentages (D) were analyzed using the same samples. Different letters indicate significant differences between different tissues (p<0.05, one-way ANOVA by Tukey’s HSD post-hoc tests).