Figure 6. The short styles of jasmonate-deficient irAOC plants result from disturbed DTG malonylation status.
(A) DTG malonylation percentage (mean + SE; n = 5) was calculated based on DTGs of styles dissected from flowers one day before anthesis. (B) Style lengths (mean + SE; n = 10) were measured from freshly opened flowers of VIGS-EV and VIGS-MaT1 silencing in the background of two stably transformed lines, EV or irAOC, as indicated. (C) Malonylation percentages (mean + SE, n = 3) were calculated based on DTGs in styles dissected from flowers one day before anthesis of the four genotypes as described in (B). (D) Mean style lengths (mean + SE; n = 10) were measured from freshly opened flowers of the four genotypes, which had been treated with lanolin paste with MeJA (MeJA) or only lanolin paste as control (Con), 2 days previously. (E) Style lengths (mean + SE; n = 5) were measured from freshly opened flowers of the four genotypes, which were from crosses of EV or irAOC with EV or irGGPPS. (F) IAA levels (mean + SE; n = 5) were analyzed from the same samples as in (A). (G) IAA (mean + SE; n = 3) was quantified from in vitro enzyme activity assay products, in which the enzyme activity is from crude protein extracted from styles as in (A). (H) Style lengths (mean + SE; n = 10 – 16) of freshly opened irAOC flowers which had been injected with 0.1% DMSO aqueous solutions with 10 µM IAA (IAA) or only 0.1% DMSO aqueous solution as controls (Con) 2 days before. Asterisks indicate significant differences between different genotypes or treatments (***p<0.001; Student’s t-test). Different letters indicate significant differences among different plant lines or treatments (p<0.05, one-way ANOVA followed by Tukey’s HSD post-hoc tests).
Figure 6—figure supplement 1. DTG malonylation status and style length are affected by silencing a JA biosynthesis gene (AOC), and are partly recovered by exogenous applications of MeJA.
