Figure 5. H2BEGFP reporter accumulates in cardiac myocytes in vivo.
EGFP is detected and quantitated in nuclei of immunolabeled CKH2B hearts treated for one day (A) or four days (B) with doxycycline (red=wheat germ agglutinin, green=H2BEGFP, blue=myosin light chain 2v (MYLC2V), gray=DAPI). Quantitation of the increase in EGFP+ cells per total nuclei (C), EGFP+ myocytes per total nuclei (D) and EGFP+ myocytes per total myocytes (E) represented in histograms. n=3 hearts, minimum 10 fields of view per heart.