Table IV.
List of antibodies for western blot analysis.
| Protein | Vendor | Cat. no. | Dilution | Incubation |
|---|---|---|---|---|
| PTP1B | Abcam (Cambridge, UK) | ab52650 | 1:1,500 | RT, 1 h |
| p-IR | Abcam | ab5681 | 1:2,000 | RT, 1 h |
| p-IRS1 | Abcam | ab66153 | 1:3,000 | RT, 1 h |
| p-PI3K | Abcam | ab182651 | 1:2,000 | RT, 1 h |
| p-AKT | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) | sc-81433 | 1:2,000 | RT, 1 h |
| GLUT1 | Abcam | ab115730 | 1:2,500 | RT, 1 h |
| GLUT4 | Santa Cruz Biotechnology, Inc. | sc-53566 | 1:2,000 | RT, 1 h |
| GAPDH | Aksomics (Shanghai) Biotechnology Co., Ltd. (Shanghai, China) | KC-5G5 | 1:10,000 | RT, 1 h |
| HRP Goat anti-Rabbit IgG | Boster Biological Technology Co., Ltd. (Wuhan, China) | BA1054 | 1:20,000 | RT, 40 min |
| HRP Goat anti-Mouse IgG | Boster Biological Technology Co., Ltd. | BA1051 | 1:20,000 | RT, 40 min |
PTP1B, protein-tyrosine phosphatase 1B; IR, insulin receptor; IRS, insulin receptor substrate; PI3K, phosphoinositide 3-kinase; AKT, serine/threonine protein kinase; GLUT1, glucose transporter 1; GLUT4, glucose transporter 4; p-, phosphorylated; RT, room temperature.