Figure 1.

Effects of CPT on cell viability and apoptosis of NSCLC cells. (A) Viability of two non-small cell lung cancer cell lines, H1299 and H460, was determined by trypan blue exclusion assay. (B) Alterations in the expression levels of apoptosis-associated proteins and the DNA damage-sensor γH2AX in H1299 cells, as determined by western blotting. GAPDH was used as an internal control to ensure equal loading. Data are presented as the means ± standard deviation of at least three independent experiments. a vs. a, P>0.05; a vs. b, P<0.05; a vs. c, P<0.001. γH2AX, phosphorylated-H2A histone family, member X (Ser¹³⁹); Bax, B-cell lymphoma 2-associated X protein; CPT, camptothecin; PARP, poly (ADP-ribose) polymerase.