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3-MA treatment enhances the expression levels of apoptotic and DNA damage-associated proteins in CPT-resistant H1299 cells. The expression levels of apoptotic and DNA damage-associated proteins were detected in H1299 cells treated with various concentrations of 3-MA in the presence of 0.5 µM CPT by western blotting. β-actin was used as an internal control to ensure equal loading. γH2AX, phosphorylated-H2A histone family, member X (Ser139); 3-MA, 3-methyladenine; CPT, camptothecin.
