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. 2018 Aug 31;42(5):2700–2708. doi: 10.3892/ijmm.2018.3850

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Cell proliferation and cell cycle assay after knockdown of FUT7. MHCC97 cells were transfected with FUT7 siRNAs and siNC. Cell proliferation was analyzed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide assay and cell cycle was analyzed by flow cytometry. (A) Cell proliferation assay following knockdown of FUT7. (B) Cell cycle assay following knockdown of FUT7. (C) Flow cytometry profiles of MHCC97 cells following transfection. ^*P<0.05 (n=3), vs. untransfected control cells. FUT, fucosyltransferase; SLe^x, sialyl-Lewis X; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA, small interfering RNA; siNC, scramble-siRNA transfected cells; Control, untransfected cells.