Figure 8.

Effects of the combined use of GDC-0941 and fractionated dose irradiation (FD-IR) on motility. (A) After culturing G40 differentiated cells (DCs) overnight, cells were either left untreated or treated for 30 min with 0.625 µM GDC-0941 (GDC). This was followed by the introduction of a single wound, represented by the white lines. Twenty-four hours time-lapse microscopy was initiated 30min after wounding. (B) Cells were irradiated with three times 2 Gy (Irradiation) or not (Control) and, after culturing G40 DCs overnight, were either treated for 30 min with 0.625 µM GDC-0941 (Irradiation + GDC) or not. This was followed by the introduction of a single wound, represented by the white lines. At 24 h time-lapse microscopy was initiated 30 min after wounding. In (A and B) one representative result of two independent experiments performed in duplicate is depicted. (C) Irradiated (3x2 Gy) and non-irradiated cells were allowed to grow for 24 h after seeding and were treated with GDC-0941 1 h before recording via time-lapse microscopy for another 24 h. Cell velocity was examined in 8-h intervals using ImageJ software. The mean of two experiments performed in duplicate is shown (n=40). Statistical significance was assessed by two-way ANOVA (^*P≤0.05; ^**P≤0.01; ^***P≤0.001).