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. 2018 Aug 9;53(5):1857–1868. doi: 10.3892/ijo.2018.4519

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Copyright: © Cao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Investigation of the role of NFYA in diffuse and intestinal-derived GC cells. (A) Western blot analysis of NFYA knockdown by siRNA in MGC803 and SGC-7901 cells. (B) CCK-8 assay of MGC803 (upper panel) and SGC-7901 (lower panel) cell growth in the presence of siNFYA; the growth curve is presented on the left, and the fold change at day 3 is presented on the right. (C) Colony formation assays of MGC803 (left panel) and SGC-7901 (right panel) in the presence of siNFYA; representative colony formation results are presented in the upper panel, and the statistical results are presented in the lower panel. All assays were performed in triplicate. ^**P<0.01; ^***P<0.001. NFYA, nuclear transcription factor Y subunit α; siCon, control siRNA; siNFYA, NFYA siRNA; siRNA, small interfering RNA.