Figure 2.

Validation of the expression of ARHGEF7 in colorectal adenocarcinoma cell lines with ARHGEF7 overexpression or knockdown. (A) ARHGEF7 was upregulated by ARHGEF7-expression LV particles in HCT116 cells. RT-qPCR and western blot analyses were used to evaluate the upregulation of exogenous ARHGEF7 in HCT116 cells. (B) ARHGEF7 was downregulated by shRNA-ARHGEF7 LV particles in LoVo cells. RT-qPCR and western blot analyses were used to assess the inhibitory efficiency of four designed ARHGEF7 shRNA LV particles and control. Expression of ARHGEF7 in control cell was set to 1. Relative expression levels of ARHGEF7 in these cells are shown in the diagram. ARHGEF7-shRNA-Seq2 reduced ARHGEF7 expression by >90% and was used for subsequent experiments due to its highly effective inhibitory efficiency. ARHGEF7, Rho guanine nucleotide exchange factor 7; shRNA, short hairpin RNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LV, lentiviral.