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. 2018 Aug 14;42(5):2403–2414. doi: 10.3892/ijmm.2018.3822

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Reverse transcription-quantitative polymerase chain reaction and western blotting were performed in rDFSCs following 1, 2, 3 and 4 weeks of culturing in osteogenic induction medium to detect the expression of Nkd2, β-catenin, Runx2 and OCN. rDFSCs harvested prior to the addition of osteogenic induction medium (at 80% confluence) served as controls. (A) Expression of Nkd2 mRNA was upregulated in rDFSCs following culturing in osteogenic induction medium, consistent with the expression of β-catenin, Runx2 and OCN. (B) Expression of Nkd2 protein was upregulated in rDFSCs following culturing in osteogenic induction medium, consistent with expression of β-catenin, Runx2 and OCN. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. values at week 0. rDFSC, rat dental follicle stem/progenitor cell; Nkd2, naked cuticle homolog 2; Runx2, runt-related transcription factor 2; OCN, osteocalcin; OD, optical density; NS, not significant.