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. 2018 Aug 14;42(5):2403–2414. doi: 10.3892/ijmm.2018.3822

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Osteogenic differentiation ability of Nkd2-silenced rDFSCs. (A) Alizarin Red staining revealed the reduced formation of mineralized nodules by Nkd2-silenced rDFSCs following culturing in osteogenic induction medium for 7 days. (B) ALP activity was blocked in Nkd2-silenced rDFSCs. (C) A simple western size assay revealed that Col-I and ALP protein levels were significantly lower in si-Nkd2-transfected rDFSCs compared with in si-NC-transfected rDFSCs subsequent to culturing in osteogenic induction medium for 7 days. ^*P<0.05 and ^***P<0.001 with comparisons shown by lines or vs. si-NC. OD, optical density; si-, small interfering RNA; Nkd2, naked cuticle homolog 2; NC, negative control; NS, not significant; ALP, alkaline phosphatase; Col-I, collagen type I; Runx2, runt-related transcription factor 2; rDFSC, rat dental follicle stem/progenitor cell.