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. 2018 Aug 14;42(5):2403–2414. doi: 10.3892/ijmm.2018.3822

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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si-Nkd2 transfection reduced activity of the Wnt/β-catenin pathway. (A) Luciferase activity at 72 h after transfection was significantly lower in si-Nkd2-transfected rDFSCs compared with in si-NC-transfected rDFSC. (B) Western blotting revealed that the expression of β-catenin, cyclinD1 and c-Myc was signficantly lower in si-Nkd2-transfected rDFSCs compared with in si-NC-transfected rDFCs. ^*P<0.05, ^**P<0.01 or ^***P<0.001 with comparisons shown by lines or vs. the si-NC group. TCF, LEF, si-, Nkd2, CR, Cignal reporter; CN, Cignal negative control; NC, negative control; CP, Cignal positive control; rDFSC, rat dental follicle stem/progenitor cell.