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. 2018 Aug 14;42(5):2403–2414. doi: 10.3892/ijmm.2018.3822

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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A simple western size assay demonstrating the levels of ALP, Runx2 and β-catenin proteins in Wnt3a- or mock-treated si-Nkd2-transfected rDFSCs. si-Nkd2-transfected rDFSCs were treated with Wnt3a or mock-treated and cultured in osteoblast induction medium for 7 days. Cells were harvested, and the protein levels of ALP, Runx2 and β-catenin proteins were analyzed by a simple western size assay. Significantly lower levels of ALP, Runx2 and β-catenin proteins were observed in si-Nkd2-transfected rDFSCs, and higher levels of proteins were observed in Wnt3a-treated cells compared with the control. rDFSCs cultured in osteoblast induction medium served as the control. ^*P<0.05 and ^***P<0.001 with comparisons shown by lines. NS, not significant; si-, small interfering RNA; Nkd2, naked cuticle homolog 2; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; rDFSC, rat dental follicle stem/progenitor cell.