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. 2018 Sep 6;42(5):2665–2675. doi: 10.3892/ijmm.2018.3861

Figure 7.

Figure 7

Effects of p38, ERK and JNK inhibitors on 1,5-diCQA-induced melanin synthesis. B16 cells were pre-incubated with SB203580 (10 µM) for 2 h prior to the addition of 1,5-diCQA (100 µM), and then (A) incubated for 48 h for the measurement of melanin content or (B) incubated for 24 h for the measurement of TYR activity. (C) B16 cells were pre-incubated with PD98059 (10 µM) for 2 h prior to the addition of 1,5-diCQA (100 µM), and then (C) incubated for 48 h for the measurement of melanin content or (D) incubated for 24 h for the measurement of TYR activity. B16 cells were pre-incubated with SP600125 (10 µM) for 2 h prior to the addition of 1,5-diCQA (100 µM), and then (E) incubated for 48 h for the measurement of melanin content or (F) incubated for 24 h for the measurement of TYR activity. Each percentage value in the treated cells was calculated with respect to that in the control cells. Values are expressed as the mean ± standard deviation of three separate independent experiments. **P<0.01 and ***P<0.001 vs. untreated control group. ##P<0.01 and ###P<0.001 vs. single drug treatment group. P38, p38 mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; 1,5-diCQA, 1,5-dicaffeoylquinic acid.