Table II.
Primers used for the amplification of the Pre-S/S genes in hepatitis B virus.
| Primer set and namea | Sequence (5′-3′) | Position (nt) | Fragment size (bp)b | Sequencing primer |
|---|---|---|---|---|
| IF | ACCWTATWCYTGGGAACAA | 2,819-2,837 | 1,554 | No |
| IR | TCAGCAAAYACTYGGCA | 1,190-1,174 | No | |
| IaF | ACCWTATWCYTGGGAACAA | 2,819-2,837 | 981 | Yes |
| IaR | GAYGAYGGGATGGGAATACA | 617-598 | Yes | |
| IbF | GACTYGTGGTGGACTTCTC | 251-269 | 939 | Yes |
| IbR | TCAGCAAAYACTYGGCA | 1,190-1,174 | Yes |
a
IF and IR refer to a pair of primers used in first-round amplification; Ia (IaF, IaR) and Ib (IbF, IbR) were used to amplify two fragments in the second-round amplification.
b
With reference to NC_003977 from National Center for Biotechnology Information. F, forward; R, reverse.