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Cultured hPRE cells. Primary (P0) hRPE cells were big irregularly shaped cells containing a great amount of pigment granules. With the progression of the cell passage, hRPE cells became smaller fusiform cells with less pigment granules. Cell viability reached a peak value in the fourth generation (P4) of hRPE cells, at which regularly shaped cells of similar size were observed (scale bar, 20 μm). hRPE, human retinal pigment epithelium.
