Figure 10.

Scanning electron microscopy demonstrated the extracellular variations in the cytoarchitecture. Treatment with 300 μmol/l t-BH of 6 h led to atrophy and permeabilization of the cell membrane. Microvilli (black arrows) and pseudopodia were abundant in the control group, however these had mostly vanished in the t-BH group. Cells turned spherical and began to atrophy (white arrow) and abscise. By contrast, the cytoarchitecture of hRPE cells in the DHA group was close to normal. In the fluoxetine co-treatment group, the majority of hRPE cells collapsed as expected (scale bar, 5 μm). hRPE, human retinal pigment epithelium; t-BH, tert-butyl hydroperoxide; DHA, docosahexaenoic acid.