Figure 11.

Transmission electron microscopy demonstrated the changes inside human retinal pigment epithelium cells. After treatment with 300 μmol/l t-BH for 6 h, the majority of normal intracellular structure disappeared: Chromatin condensated, apoptotic bodies were formed (black arrow), cytoplasm dyeing decreased, mitochondrial edema emerged, and endoplasmic reticulum and Golgi bodies were destroyed. Pretreatment with 10 μmol/l DHA evidently reduced this collapse, although deformation of nuclei and cytoplasmic organoids were still observed. However, co-treatment with 70 μmol/l fluoxetine resulted in severe damage of the intracellular cytoarchitecture (scale bar, 2 μm). t-BH, tert-butyl hydroperoxide; DHA, docosahexaenoic acid.