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. 2018 Aug 8;42(5):2584–2594. doi: 10.3892/ijmm.2018.3813

Figure 5.

Figure 5

Immunofluorescence analysis revealed the variations in Bax and Bcl-2 expression. Treatment with 300 μmol/l t-BH for 3 h evidently increased Bax and decreased Bcl-2 in human retinal pigment epithelium cells under oxidative stress. Introduction of 10 μmol/l DHA for 24 h before t-BH exposure restrained these variations. However, co-treatment with 70 μmol/l fluoxetine for 24 h alongside DHA reversed the effects caused by DHA (scale bar, 10 μm). t-BH, tert-butyl hydroperoxide; DHA, docosahexaenoic acid.