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. 2018 Aug 8;42(5):2584–2594. doi: 10.3892/ijmm.2018.3813

Figure 8.

Figure 8

RT-PCR demonstrated the variations in Bax, Bcl-2, caspase-3 and αB-crystallin mRNA levels. [Groups: 1=control, 2=(3 h) t-BH, 3=(3 h) t-BH+DHA, 4=(3 h) t-BH+DHA+fluoxetine]. Following treatment with 300 μmol/l for 3 h, the levels of these mRNAs were tested using RT-PCR. The fold changes in the different groups of human retinal pigment epithelium cells revealed that the oxidative stress enhanced the transcription of Bax and caspase-3, while it reduced that of Bcl-2, and slightly increased that of αB-crystallin. Thus, TREK-TRAAK two-pore domain potassium channels inhibited the oxidation by increasing the transcription of Bax and caspase-3, and decreasing the transcription of Bcl-2, yet further promoting the transcription of αB-crystallin. Data are presented as the mean ± standard error (n=3) of triplicate experiments. **P<0.01 vs. control; ##P<0.01 vs. t-BH or DHA + fluoxetine groups. t-BH, tert-butyl hydroperoxide; DHA, docosahexaenoic acid; RT-PCR, reverse transcription-polymerase chain reaction.