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. 2018 May 25;63(4):E123–E129.

Figure 3. SphK2 plays a role in cargo sorting into exosomal vesicles in an activity-dependent manner.

Figure 3

K562 cells transiently transfected with a vector encoding either siRNA-resistant HA-SphK2 or HA-SphK2(G248D) together with control or SphK2-siRNA were cultured for 48 hr. Exosomes were prepared as in Fig. 2B and analyzed for cargo contents by immunoblot analysis using anti-flotillin 2 antibody (A). K562 cells were cultured in exosome-depleted medium for 12 hr in the absence or presence of either 10 μM DMS or 50 μM HACPT. Exosomes were purified from the media and analyzed for cargo contents by immunoblot analysis using anti-TfR1, anti-HSP70, and anti-flotillin 2 antibodies (B).