Fig. 1. HG reduces the expression of TRPV4 in HEI-OC1 cells. HEI-OC1 cells were cultured under euglycemic, hyperglycemia, and osmotic control conditions for 48 h, and qRT-PCR (A) and Western blot (B) were used to analyze the expression of TRPV4 mRNA and protein. Results are representative of three independent experiments. ^*Indicates differences from controls at p<0.05. TRPV4, transient receptor potential vanilloid sub type 4; HG, high glucose.