Fig. 2. The effect of reagents on the expression of TRPV4. (A) The relative expression of TRPV4 mRNA in high glucose cultured HEI-OC1 cells after treatment with HC-067047 (HC) or GSK1016790A (GSK), compared with the untreated control, was determined by qRT-PCR. (B) Western blotting was used to analyze the expression of TRPV4 protein in HEI-OC1 cells after treatment with HC-067047 or GSK1016790A. Data are representative of three experiments. ^*Indicates differences from euglycemic controls at p<0.05, ^†Indicates differences from euglycemic controls at p<0.01, ^‡Indicates differences from untreated controls at p<0.05, respectively. TRPV4, transient receptor potential vanilloid sub type 4.